Met4 and Gcn4 association with promoters. Cells expressing Myc-tagged Met4 or Gcn4 (Y1526 & Y1578) were grown and starved as in Fig. 1 . Association with the indicated genes was measured by ChIP with antibodies against the Myc-tag. DNA was quantified by qPCR using primers specific for the promoter regions of ATG1 , ATG8 , ATG9 , ATG41 , and MET16 and for the middle region of IME2 ORF . MET16 and IME2 were used as positive and negative controls, respectively. Data are mean ± SEM ( n = 4). Statistical significance compared with t0 was determined by two-way ANOVA with Geisser-Greenhouse correction followed by Dunnett’s multiple comparisons test. P values for Met4: **** p < 0.0001; *** p = 0.0005; ** p = 0.0026, 0.0013, 0.0013, 0.0085, and 0.0096; * p = 0.0426, 0.0146, 0.0363, 0.0278 and 0.0136; ns = 0.16, 0.95, 0.07 and 0.11 (from left to right). For Gcn4: ** p = 0.0076, 0.0093, 0.0048, 0.0029 and 0.0029; *p= 0.0417, 0.0234, 0.043, 0.0126; ns =0.481, 0.133, 0.556, 0.169, 0.999, 0.222 and 0.094. Source data are provided with this paper.
