Bar Plot from Scientific Research

GFP-Atg8 processing assay. A prototrophic strain expressing GFP-Atg8 (Y1727) was grown into SF-medium supplemented with 0.1 mM Met and transferred into SF-medium without sulfur supplementation or into a derived medium lacking ammonium and amino acids except for 0.1 mM Met. Cells were collected before (t0), and 2, 4, and 6 h after starvation. GFP-Atg8 expression and GFP release are relative to the 6-h time point in no sulfur. Data are mean of n = 2 independent experiments.

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