Bar Plot from Scientific Research

RNA-sequencing analysis. Cells (BY4742) were grown overnight in SF-medium supplemented with 0.1 mM Met and starved as described in Methods. Cells were collected by centrifugation before (t0) and 40, 80 and 120 min after the shift. RNA was extracted and processed for RNA-sequencing as described in Methods. The upper graph indicates maximum log2-fold change (FC) values (plain blue circle) and mean normalized counts (gray bars) calculated by the DESeq2 package for the 36 ATG genes. The lower graphs represent log2-FC values and normalized counts at the different time points for the top induced ATG genes (log2-FC > 3). Data are mean ± SD (log2-FC) or ± SEM (normalized counts), n = 3 RNA preparations from independent cultures. Source data are provided with this paper.

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