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Hybridization of interacting region 1 containing a (CAG) 4 sequence with interacting region 2 containing a (CTG) 4 sequence forms a switchback DNA, reducing fluorescence signal. Two controls were used in this study. The interacting region 2 in the first control contains two repeating units of CTG and six thymines (denoted (CTG) 2 T 6 ), and the interacting region 2 in the second control contains twelve thymines (denoted T 12 ).
#Illustration#Workflow#Hybridization#Interacting Regions#CAG Repeat#CTG Repeat#Switchback DNA#Fluorescence#Controls
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