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UNC-45 proximity proteomics under mechanical stress conditions. Proteins biotinylated by the fusion ligase UNC-45-miniTurbo in UNC-45 proximity in worms subjected to sustained body wall muscle contractions by 13 5-s blue light pulses with (iv) or without (v) ATR supplementation were pulled down with streptavidin beads and identified by mass spectrometry.
#Workflow#Illustration#Proteomics#UNC-45#Ligase#Mechanical Stress#Muscle Contractions#Mass Spectrometry#Streptavidin Beads
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