Bar Plot from Scientific Research

Results of transthiolation assays with E2, Ub and E3 mutations to probe putative proteinprotein interactions during transthiolation, quantified after normalization to wild type (WT) with indicated mutations labelled and colour-coded. Densities and models colour-coded according to a . Isosurface levels contoured at 0.470.52 ( b ); at 0.72, 0.75 and 0.71 for state 1 and 0.5, 0.6 and 0.59 for state 7 ( c , e , f ). Bars represent mean s.d of n = 3 replicates ( n = 9 and n = 6 for wild type in E2 and E3 mutations sets, respectively). Statistical differences between wild type and mutants by twotailed unpaired t -test, ***P < 0.001 (Supplementary Fig. 4 ).

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