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Reaction of the Ub-PSAN probe with E2 to form E2Ub vinyl thioether and subsequent reactions with E1 and E3 HECT to obtain stable analogues of transthiolation intermediates.
#Workflow#Illustration#Ub-PSAN Probe#E2 Enzyme#E2-Ub Vinyl Thioether#E1 Enzyme#E3 HECT Enzyme#Transthiolation Intermediates
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