Exosomes were immunomagnetically isolated from dissociated muscle tissues using anti-CD63 MNP. Isolated CD63+ exosomes were characterized by FACS for the expression of exosomal markers. Left, Representative FACS dot-plots show the proportion of muscle-isolated CFSE+ exosomes in both Exo C2C12 - and Exo myo -injected mice. Right, Representative FACS dot-plots show the coexpression of CD9 and CD81 within the CFSE+ Exo C2C12 and Exo myo populations. FACS was performed on n = 3 mice per group (pooled muscles) in n = 2 independent experiments.
