Representative Amnis ImageStream of CFSE-labelled Exo C2C12 showing the scatter plot of SSC versus CFSE intensity, which resolves three discrete populations (SpeedBeads (SB); Background (BG); Exosomes (R1), 0–200 nm) (left panel). CFSE staining is evident only in the R1 Exo C2C12 population as confirmed by CFSE, SSC and bright-field (BF) imagery (right panel). In a – d representative images from n = 3 independent experiments are shown.
