Gel from Scientific Research

SDS PAGE of the proteolytic digestion of green mEos, either alone (right lanes) or fused to I3 (SP I3 mEos ; left lanes) and at increasing concentrations of B(aCt Cy5 ) (1 10 nM). mEos is stable to proteolysis. Lack of aCt bands in B(aCt Cy5 ) lanes indicates that immobilized aCt is protected from autoproteolysis. The gel was stained with Coomassie and scanned with a Typhoon FLA9000 (GE Healthcare Life Sciences).

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