Protein Structure from Scientific Research

Top: atomic model of p97-HaloTag predicted by AlphaFold2. The relative position of the HaloTag-cPA domains (cyan) cannot be predicted accurately due to the flexibility of the C-terminal tails of p97 (red); nevertheless, they are aligned with the PAs located 16 nm away from one edge of the compartment. Bottom: the electron density map of A(p97) shows an asymmetric protein signal localized at one side of the chamber. The atomic model of p97-HaloTag fits into the protein density map only in the N-out configuration.

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