Microscopy from Scientific Research

RNA-nanostar designs are modified to include a single-stranded 5' overhang that can connect to a protein-binding moiety. Design A YFP is identical to nanostar A (Fig. 1 ) but can connect to YFP-binding aptamer (YFP apt ) (i). Created with BioRender.com . Design B STV is identical to B but can connect to either an STV-binding aptamer (STV apt ) (ii) or a biotinylated DNA oligonucleotide (Biotin DNA ) (iii). The templates of protein-binding aptamers (YFP apt -T and STV apt -T) are transcribed in synthetic cells alongside the associated RNA-nanostar templates (A YFP -T and B STV -T, respectively), while pre-synthesized Biotin DNA is encapsulated alongside template B STV -T.

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