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Polymersome in vivo biodistribution was assessed by PET/CT imaging in B16F10 melanoma-bearing C57BL/6 mice upon polymersome administration intravenously (i.v.) via lateral tail vein injection or subcutaneously (s.c.) via footpad injection. Using flow and mass cytometry, we studied polymersome uptake by specific immune cells, including the different splenic compartments, that is, white pulp, red pulp and the marginal zone.

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