Immunofluorescence analysis of MAOA and MCAM expression at the protein level in organoids treated with and without cytokines. Organoids were fixed and immunostained with the indicated antibodies at 96 h after NRG1 removal. Top: representative images of three independent experiments, scale bars 50 μm. Bottom: quantification of MAOA, MCAM, and HLA-G intensity in isolated cells. Results are expressed as fold change with respect to the average intensity in untreated organoids and represented with violin plot reporting median and quartiles. MAOA and HLA-G (left): -cytokines, n = 371 cells; +cytokines, n = 410 cells from three independent experiments. MCAM and HLA-G (right): -cytokines, n = 405 cells; +cytokines, n = 378 cells from three independent experiments. ns, not significant;****p < 0.0001 by Mann-Whitney test. See alsoFigure S6andTables S1,S4, andS5.
