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A graphic illustrating the events of a multi-stage sequential release of the same cargo (calcein dye) from the two compartments of a concentrisome. Calcein was encapsulated in both the inner liposome and intermembrane space of a concentrisome system, with the inner bilayer composed of our DSPC composition ( T m 52 C), and the outer bilayer of our DPPC composition ( T m 42 C).

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