Workflow from Scientific Research

Open access visualization of Workflow, Illustration, Active Genes, Mouse ES Cells, Intron Probes
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Active genes (208) across Chr 2 in mouse ES cells are targeted by 30 intron probes per gene. Primary probes for each gene contain four unique docking sites, and each docking site can be hybridized to a readout probe corresponding to nine pseudocolors in each readout round. The identity of each bursting gene can be determined according to the barcode after four readout (RO) rounds. The barcode is designed that the gene can be decoded even with one round of drop out.

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