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Editing using pilot SGE conditions: a template library (496 variants) coupled with sgRNA-A targeting exon 5 of BAP1 was transfected into the polyclonal (Cas9 + LIG4 ) line and cells were sampled at D5 and D11 (time points previously 10 used in SGE). More than 10% of the counts were unedited (wild type), which decreased to <1% when the clonal (Cas9 + LIG4 ) cell line (HAP1-A5) was edited using the same sgRNA and HDR homology arms with optimized SGE conditions, including a high-complexity template library (1,040 variants) sampled over five time points.

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