Bar Plot from Scientific Research

FACS analysis counts (green fluorescent protein (GFP)-positive cells) demonstrated that the HAP1-A5 clone has very high Cas9 activity (arrow), measured at 48 and 72 h after transduction with a GFP/blue fluorescent protein (BFP) activity construct (Methods: Ploidy and FACS analysis), compared to the parental Polyclonal (Cas9 + LIG4 ) line. A total of 10,00020,000 cells were analyzed for each line. Cell count percentages derived from negative-control lines with no Cas9 showed expected low levels of Cas9 activity (see Extended Data Fig. 1a and Supplementary Fig. 1a for representative FACS data).

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