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Open access visualization of Scatter Plot, Thymocytes, CD4/CD8 DN, DP, CD4 Single Positive
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(i), Thymocyte populations at consecutive developmental stages—CD4/CD8 DN, DP, CD4 or CD8 single positive (SP) of Stag2 WT Stag2 variant heterozygous females. (ii) Sanger sequencing of Stag2 WT Stag2 variant thymocyte cDNA. (iii) Allele-specific qRT–PCR of Stag2 WT and Stag2 variant thymocyte cDNA ( n = 4). (iv) Live-cell reporter assay for the representation of Stag2 WT (FITC-negative) and Stag2 variant (FITC-positive) clones in thymocyte subsets. Genotypes as in c .

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