(i), Mature CD4 T and B lymphocytes from Stag2 WT Stag2 variant heterozygous females. (ii) Sanger sequencing of cDNA. (iii) Allele-specific qRT–PCR ( n = 9). (iv) Live-cell reporter assay for the representation of Stag2 WT (FITC-negative) and Stag2 variant Atrx Luc / βGal (FITC-positive) clones in mature CD4 T cells ( n = 6, mean = 1.7 ± 1.7% FITC-positive) and B cells ( n = 6, mean = 3.3 ± 3.2% FITC-positive) at the single-cell level (red histogram). X Stag2- WT and X Stag2- WT Atrx-Luc / βGal heterozygous cells are shown as control (black histogram).
