Chemical Structure from Scientific Research

Outlined of a general scheme: an initiator oligonucleotide (black) is bound to LCAA-CPG with a Bis(NHS)PEG5 linker (pink) using NHS conjugation chemistry. The initiator harbors a deoxy- or riboinosine base (red) for recognition by E. coli endonuclease V, which cleaves the desired oligonucleotide product (blue) immediately downstream of the inosine base. The oligonucleotide product can then be isolated from the CPG solid support.

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