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Modified 3- O -allyl ether and PS 3- O -azido methyl ether RT-NTPs were evaluated by performing an initial N + 1* extension, a deblocking reaction and, if possible, an N + 2* extension. A green checkmark indicates a successful reaction, and a red cross-out indicates an unsuccessful reaction. Reactions that were not attempted are indicated by a yellow bar. Each individual cycle step was evaluated using MALDI-TOF mass spectrometry (Supplementary Figs. 11 – 13 ).

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