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A complete set (A, U, G, C) of 3- O -allyl ether NTPs were tested for enzymatic incorporation and deblocking using a liquid bulk phase reaction scheme, where N is the length of the initiator, N + 1* is the extension intermediate with the 3- O -allyl ether group as represented by the asterisk, and N + 1 is the deblocked product for each base.

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