Chemical Structure from Scientific Research

Three primary components are required for carrying out an enzymatic extension: 3′-blocked reversible terminator nucleotides, enzymes capable of their robust and indiscriminate incorporation, and an initiator oligonucleotide. The reversible terminator group stops uncontrolled polymerization by the enzyme and limits extension to a single incorporation event. The initiator oligonucleotide may vary in terms of sequence and length. It can also be bound to a solid support or feature other modifications such as a 5′-fluorophore or functional handle.

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