Thirty key markers related to the TCR signaling network in the human Jurkat T cells were measured with or without ACE amplification and plotted as green or blue boxes, respectively. Mean ion counts for unamplified and linear amplified staining were indicated by the green and blue points with lines between them color coded according to amplification fold change. Boxplots present the first quartile (Q1), median and third quartile (Q3) in unamplified or linear amplified data. The IQR defines the distance between Q1 and Q3. The upper whisker extends from the hinge to the largest value no further than 1.5 x IQR from the hinge. The lower whisker extends from the hinge to the smallest value no further than 1.5 x IQR of the hinge. ACE enabled, on average, 17-fold and a maximum of 41-fold signal amplification. The signal from p-AKT (p-T308) was analyzed with an additional ACE branching amplification (shown as a red box) with 15-fold higher p-AKT (p-T308) counts after the branching amplification.
