Density Plot from Scientific Research

Jurkat cells treated with anti-CD3 and anti-CD28 antibodies, and subsequently crosslinked with anti-mouse IgG antibody to activate TCR signaling. Cells were harvested at 0, 0.5, 2, 5, 10, 15, 30 and 60 min after TCR activation. Linear amplification allowed the quantification of key signaling nodes in the TCR network during the 1-h TCR stimulation timecourse. Negative control p-SMAD2 did not show differential phosphorylation levels as measured with ACE.

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