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Circos plot shows 73 pairs of strong signaling relationships in the TCR signaling networks as detected with the BP-R 2 method on the TCR stimulation timecourse data measured by ACE (inner layer). The middle layers show the coefficients of variation of measured phosphorylation sites at all timepoints following TCR stimulation and the signal amplitude for each phosphorylation site. The outer layer demonstrates the signaling trajectory for each marker, characterized by normalized mean ion counts across all timepoints during the TCR stimulation timecourse. Cells used in these analyses were in G1 phase to avoid cell cycle confounding effects. Experiments were performed in biological replicates to confirm reproducibility.

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