Table Image from Scientific Research

Yields achieved using circular CLUSTER guide RNAs containing a 100-nt-long antisense part split into a targeting sequence (20 nt) and four RSs (each 20 nt). The basic design (column circular CLUSTER) does not contain bystander solutions. The other guide RNAs contain GU wobbles at positions 49, 27 and +48, as well as no bystander solution, GA mismatch or depleted U at position 5.

DOI