Editing heat maps of the LEAPER guide RNA-binding sites within the indicated transcripts: BMPR2. The basic design column, LEAPER, lacks G.A mismatches and wobble base pairs. The other guide RNAs contain either G.A mismatches or G.U wobbles at G.U-amenable sites or a combination of both solutions at all bystander sites. In the latter case, G.A mismatches are placed at sites not amenable to G.U wobbles. The triplet context for each listed editing event is given with the target A highlighted in green and all off-target A bases in blue. The position of each site is given relative to the transcript and the target A (0 position). Editing was performed with plasmid-borne guide RNA and target in HeLa cells (endogenous ADAR). Data are shown as the mean editing percentage s.d. of n = 5 biological replicates.
