Scatter Plot from Scientific Research

Schematic of the human genome-wide TCR-MAP screen of the a3a TCR + SrtA-Jurkats and peptide enrichment results. The 90-aa peptides tiling across the human proteome with 22-aa overlap were expressed in HLA-A*01:01 + G 5 -target cells. These cells were screened with the evolved MAGEA3 TCR. Each dot represents one peptide, with the y axis plotting the -log 10 adjusted P values determined by Mageck and the x axis indicating the geometric mean of the enrichment of the peptide across three replicates. Fold enrichment is defined as the ratio of the abundance of the peptide in the sorted population relative to the input library. Peptides highlighted in red contain the epitope from MAGEA3. The remaining colored dots indicate additional cross-reactive fragments that were validated. Validated peptides that scored in the a3a TCR screen are listed with the antigenic epitope colored.

DOI