Bar Plot from Scientific Research

EpitopeID ranking of potentially cross-reactive peptides. Saturation mutagenesis heatmap values were used to rank 9-aa peptides tiling the entire human proteome. Scored peptides were then filtered for HLA-A*01:01 binders by NetMHC and listed in descending order of the calculated stimulation potential score. Peptides 6-10 were tested for reactivity by performing peptide pulsing experiments in HLA-A*01:01 + G 5 -target cells and the CD69 upregulation of a3a SrtA-Jurkats is reported. **** P < 0.0001 for each group relative to the no-antigen control, determined by a two-tailed t -test. Each dot represents a different biological replicate, where error bars indicate the mean and s.d. Peptide pulsing experiments are representative of n = 3 independent biological replicates.

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