Workflow from Scientific Research

Workflow of TCR-MAP antigen discovery pipeline. Engineered target cells were infected with a lentivirus encoded peptide library, selected with antibiotics and cultured with SrtA-Jurkats expressing TCRs of interest in the presence of LPETGbiotin substrate. After 812 h, biotinylated target cells were isolated by fluorescence-activated cell sorting (FACS), and peptide-encoding DNA sequences were PCR-amplified from gDNA. Sequencing and analysis of enriched reads relative to the input library enabled the calculation of adjusted P values to identify enriched peptides.

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