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Design of the SIINFEKL epitope saturation mutagenesis library. Each position was substituted to each of the 19 alternative amino acids. In addition to the epitope, the two amino acids that were N terminal and C terminal to the epitope were examined. Each mutant epitope was expressed as 56-aa peptide tiles, introduced into H2-K b + G 5 -target cells and screened using OT-I TCR + SrtA-Jurkats using TCR-MAP.

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