Bar Plot from Scientific Research

The effect of knockdown of SURF ( c ) and EJC ( d ) member proteins on the RORC RNA switch reporter gene expression, relative to a scrambled sequence. The individual genes were knocked down using the CRISPRi system in both the reference and the scrambled cell lines, then the change of reporter gene expression was measured using flow cytometry ( n = 2 replicates). The bar plots show the ratio of the expression of the scrambled sequence to that of the wild-type sequence of the RORC RNA switch. P values were calculated using the two-sided Students t -test.

DOI