Protein Structure from Scientific Research

Three structural classes of the refolded RORC 3 mRNA element as determined on cryo-EM processing, with RNA-like features (top). Further cryo-EM imaging and 3D classification of the 77-GA mutant (middle) and 117-AC mutant (bottom) indicate that Class A is present in wild-type and 77-GA samples but absent from the 117-AC sample, and Class B is conversely present in wild-type and 117-AC samples but absent from the 77-GA mutant. Class C is common to all three samples. We thus assign Class A as the conformation 1 state, and Class B as the conformation 2 state. We propose Class 3 to represent a partly folded intermediate that is not disrupted in the mutated constructs.

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