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Schematic representation of the experimental schedule. Eight-week-old Krt32 wildtype (WT) and knockout (KO) C57BL/6J mice (n = 8 mice/group) were subcutaneously injected with TNF at a dose of 6 g/kg/day body weight into their shaved dorsal skin for a duration of 48 h. The dorsal skin of Krt32 ( -/-) mice exhibited pronounced thickening and extensive yellow scaling, resembling the cutaneous manifestations observed in human patients with pityriasis rubra pilaris (PRP). Skin, hair, and nail samples were collected for RNA-seq, H&E staining, transmission electron microscopy (TEM), and scanning electron microscopy (SEM) analysis.

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