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Schematic diagram of the procedure to identify KRT32 interaction proteins in primary keratinocytes cells. Escherichia coli (E. coli) extracts containing GST (negative control) or GST-KRT32 proteins were incubated with cell lysates of primary keratinocytes cells and glutathione-Sepharose beads to pull down GST complexes, and the bound proteins were then analyzed by mass spectrometry.
#Workflow#Flowchart#Illustration#KRT32#Interaction Proteins#Primary Keratinocytes#E. Coli#GST#Cell Lysates#Glutathione-Sepharose Beads#Mass Spectrometry
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