Top: naive CD8+T cells were activated with αCD3, αCD28, and IL-12, as well as IL-2, IL-7, and IL-15. Cells are cultured with 1 ng/mL IL-12 unless otherwise indicated. Bottom: representative lineages demonstrating clonal heterogeneity inTcf7-YFP silencing: image snap shots (left), lineage trees (middle), and reporter intensity (area × median YFP fluorescence) over time for each track (right), with the first cell division marked by a vertical dashed line. Cell borders in snapshots are colored and labeled to match their corresponding leaves in the lineage trees. Scale bar, 10 μm. Lineage trees and tracks are colored by HMM-derived promoter state, outlined in (B).
