Growth trends of S. cerevisiae YPH500 nfuΔ::LEU2 isu1Δ::URA3 mis1Δ::HIS3 (yeast only strain), S. cerevisiae YPH500 nfuΔ::LEU2 isu1Δ::URA3 mis1Δ::HIS3 -SynJEC3, S. cerevisiae YPH500 nfuΔ::LEU2 isu1Δ::URA3 mis1Δ::HIS3 -SynYLG6 chimeras. Cells (3.00 × 10 3 ) from each round were spotted on subsequent selection medium as described in the methods section and the final number of cells/spot on plate were determined after 48 h of growth ( n = 5 technical replicates; data are presented as mean values + /− SEM).
