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The sources of inorganic carbon (C i ) utilized by air-grown Syn7942 are 13 C-labeled HCO 3 - in the culture medium and atmospheric CO 2 . 13 C-labeled HCO 3 - is transported into the cell and is converted into CO 2 in the carboxysome, where it is subsequently converted into organic carbon via C 3 fixation. 13 C-labeled sugars are exported by Syn7942 and act as yeast feedstock and are metabolized further. Ethanolic extracts of the yeast/cyanobacteria chimera metabolome are analyzed by LC/MS to search for yeast metabolites labeled with 13 C originating from C i . Panel A was created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.

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