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Targeted analysis ( n = 1) of unlabeled PEP (M +, blue) was measured at m/z 169, as well as 13 C-labeled PEP isotopes of the precursor measured at three charged states: M + 1 (M + 13 C, m/z 170, orange), M + 2 (M + 2 13 C, m/z 171, gray), and M + 3 (M + 3 13 C, m/z 172, yellow). Visual representation of total ion chromatogram (TIC) scaled based on relative isotope distribution % of PEP and its charged states. Extracted ion chromatograms are included in Supplementary Fig. 6 .
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