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Our platform: We use suicide plasmid-based strategy to engineer cyanobacterial endosymbionts, SynYLG6 strains, such that they can secrete glucose as well as ATP. S. cerevisiae mutants, deficient in ATP synthesis by oxidative phosphorylation under defined photosynthetic selection conditions, are used as the host strains. Engineered cyanobacteria strains, SynYLG, are then introduced into the yeast cells by a cell fusion process that is developed and optimized (see “Methods”). The yeast/cyanobacterial chimera are selected under defined photosynthetic selection conditions where the cyanobacterial endosymbionts provide glucose and ATP to the mutant S. cerevisiae host cells. Figure was prepared using BioRender.

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