Volcano plot displaying tandem mass tag mass spectrometry (TMT-MS) affinity purifications of (b) 3XFLAG-Swi6-K. Samples were normalized to untagged Swi6 and the relative abundance over 3XFLAG-Swi6-WT were analyzed using a two-sided t-test. Shaded boxes mark a p-value = 0.01 (horizontal) and a 1.25-fold enrichment over the 3XFLAG-Swi6-WT reference (vertical) (N = 3), only interactions detected with > 1 peptide are included in the dataset. Proteins are color-coded as upregulated interactions (orange), downregulated interactions (purple), unenriched interactions (gray), and relevant heterochromatin regulators are labeled. The full dataset of interacting proteins can be found in Supplementary Data 1.
