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Schematic depicting single-molecule microscopy live-cell tracking workflow. PAmCherry-Swi6 molecules are photoactivated (406 nm) then imaged and tracked until photobleaching (561 nm, 25 frames/sec). The cycle is repeated 10–20 times/cell.
#Workflow#Illustration#Single-Molecule Microscopy#Live-Cell Tracking#PAmCherry-Swi6 Molecules#Photoactivation#Photobleaching
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