Naive CD8+T cells were isolated from Tcf7-YFP reporter mice, then cultured using this ex vivo system. Flow cytometry plots show analysis of cultured cells during initial stimulation for 2 days (left) and continued stimulation to day 5 (middle), or after stimulation withdrawal (removal of CD3/CD28 after day 2 and IL-12 after day 3) (right). From top to bottom: CellTrace Violet (CTV) dilution; Tcf7-YFP histograms (shaded histograms are from Tcf7-YFP+/- mice and open histograms are from wild-type mice); flow plots of CD44 against Tcf7-YFP and of CD62L against Tcf7-YFP. Data are from a single experiment representative of at least 3 independent experiments.
