A cassette directing the expression of FAM122A WT and SLiM-MT was stably introduced in HEK293 FAM122A KO cells. Expression of FAM122A wildtype but not SLiM mutant rescued the proliferation defect. Statistical analyses of proliferation curves comparing rescues to FAM122A knockout cell lines was conducted using a Friedman’s test (two-sided) of the last 10 measurements. HEK293 vs KO p value < 0.0001 (****), HEK293 WT Clone 1 p value = 0.0436 (*), HEK293 WT Clone 2 p value = 0.0002 (***), HEK293 SLiM MUT Clone 1 p value = 0.2640 (ns). FAM122A WT and SLiM mutant reconstitution are show in separate graphs compared to the same controls to more clearly visualize partial rescue (green curves) vs. absence of rescue with the SLiM mutant (magenta curve). Data are presented as mean values +/- SEM. Source data are provided as a Source Data file.
