Violin plots of CD45 (blue) and SHP2 (green) exclusion values comparing clone 19 and PD-L1 mediated contacts: WT-clone 19 (N = 193 cells), Y>F-clone 19 (N = 173 cells), WT-PD-L1 (N = 136 cells), and Y>F-PD-L1 (N = 168 cells), for cells expressing either PD-1WT (WT) or PD-1Y>F (Y>F). In (C) and (D), the Kruskal-Wallis test with Dunn's multiple comparison follow-up testing was used to compare each group to the WT control.