Microscopy from Scientific Research

Mask-based analysis of CD45 exclusion from regions of high antibody fluorescence intensity for cells treated with clone 19 (N = 204 cells) and clone 2 (N = 212 cells). The setting for the imaging experiments was analogous to that inFigure 3J. In the violin plots, dashed lines indicate the median, and dotted lines the quartiles. Scale bar is 5 μm. Data were combined from experiments performed over 3 separate days. A two-sample Student’s t test was used for statistical comparisons.

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