Microscopy from Scientific Research

RNAscope validation of GRM3 and LGR6 as markers of AQP4 + neocortical and TH astrocytes, respectively (bold markers in c ). Representative images (left) showing AQP4 transcripts (blue puncta) and GRM3 or LGR6 transcripts (red puncta). Scale bars, 20 m. Quantification (right) was performed using two-tailed unpaired Students t -tests; **** P < 0.0001. Each dot represents an individual cell, pooled from eight samples (four individuals; each with one PFC and one thalamus sample). GRM3 : n = 37 (PFC) and n = 23 (TH) cells; LGR6 : n = 17 (PFC) and n = 23 (TH) cells.

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