Workflow from Scientific Research

Open access visualization of Workflow, Illustration, Memory Assay, Optogenetic Activation, Experimental Flies
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Bottom, timeline for 3-h assay of memory with optogenetic activation for four groups of flies on a trackball. Experimental (MB085C/UAS-CsChrimson-tdT) but not control (MB085C/+) flies expressed the CsChrimson opsin in MBON-γ1pedc>α/β. Each genotype was split into groups that did (light-on) or did not (light-off) receive optogenetic illumination (30 pulses of 0.5-s red light; red shading) during odour presentation. The protocol is that of Fig. 3a but with memory testing 5 min and 3 h after conditioning. Top, timelines for individual training and testing bouts. Odours (ACV and ethyl acetate) were initially attractive and counterbalanced as CS + and CS − across 12 flies. The results are shown in l and m.

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